LAVABLUETM: THE ALTERNATIVE FLUORESCENT STAIN TO COLLOIDAL COOMASSIE
PRODUCT FEATURES
- Simple and quick protocols – 2.5 hr
- Linear quantification – >2.5 orders of linear dynamic range
- Compatible with standard imaging equipment (Polaroid® or CCD cameras)
- Sensitive – 2.5 ng/ protein band
- MS-compatible – compatible with PMF down to 5 ng/band
- Ready-to-use – no need to prepare the stain
- Comparable in price with colloidal Coomassie
LavaBlue, Alternative Fluorescent stain to Collodial Coomassie Flyer
(2967K) 
The LavaBlue-stained 1-D SDS-PAGE gel (A) showing linearity of at least two orders of magnitudes (B). The image was obtained using a UV-based CCD camera, e.g. G:BOX (Syngene) with short wave band pass filter (450 – 550 nm). Two-fold dilution of the protein samples was made. Lane 1 contains the two target proteins, Bovine serum albumin (2nd band) and carbonic anhydrase (4 th band) at a concentration of 1280 ng/protein band, and lane 10, 2.5 ng/protein band. |
2-D gel (chicken egg yolk extract) stained with LavaBlue TM (C) and colloidal Coomassie (D). LavaBlue protocol involves 1h-fixation, 1h-staining and 0.5 h-enhancement. Coomassie protocol involves overnight staining and 2-day destaining. Image of LavaBlue-stained gel-image was obtained using a CCD camera with UV/short wave band pass filter. Colloidal Coomassie-stained gel-image was obtained |
ORDERING
LavaBlue can be ordered directly from Fluorotechnics, either by faxing your purchase order to us on +61 2 9850 8253 or via our web store.
PROTOCOL
LavaBlue Kit Protocol (183K)
MSDS
LavaBlue Kit MSDS (LB-011001) (116K)
LavaBlue Kit MSDS (LB-011005) (116K)
WHAT OUR CUSTOMERS THINK
Applimex
Click here for Applimex's Customer Testimonial. (44K)
PUBLICATIONS
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PRODUCT SUPPORT
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