FLUOROPROFILETM PROTEIN QUANTIFICATION KIT

FluoroProfile is designed for the accurate and sensitive determination of proteins.

FluoroProfile is based on Fluorotechnics proprietary fluorophore epicocconone which fluoresces intensely red on binding to proteins (Coghlan et al, 2005). Binding is reversible rendering stained protein completely amenable to mass spectrometry, N-term sequencing and to functional assays.

FluoroProfile is robust to substances that inhibit most protein assays and is particularly suited to quantifying proteins in samples prepared for 2-D and 1-D electrophoresis, IEF gels and anywhere accurate quantification of protein is needed.

PRODUCT FEATURES

• Sensitive, enabling as little as 40 ng/mL protein to be accurately quantified
• Wide range of convenient assay volumes: 100 µL - 3 mL
• Wide dynamic range, over 3-orders of magnitude
• Easy, simply mix 1 part working solution with 1 part of sample
• Convenient packaging - use FluoroProfile straight from fridge
• Quick - data can be read within 20 mins
• Assay is robust to interfering compounds such as DNA and solubilisation reagents used in 1-D and 2-DGE sample preparation
• Signal is not affected by light or temperature and remains stable for up to 6 hours
• Time-consuming heating and reduction steps of protein are not required
• The kit is free of strong solvents and acids
• Low protein-protein variability
• The kit is designed for use in a wide range of fluorescence measuring instruments
• Proteins are not precipitated or denatured and can be used in subsequent assays

FluoroProfile Protein Quantification Kit Flyer (357K)

a)

b)

Fig 1. Assay example. BSA was prepared in water at a concentration of 1.64 mg/mL and diluted four-fold to 40 ng/mL. Aliquots (50 µL) were added, in triplicate, to a 96 well plate and 50-µL aliquots of the working solution of FluoroProfile were added. The plate was left for 40 min at room temperature and then imaged on a Molecular Dynamics Typhoon. a) an image from the Typhoon scanner of the data-point cells used to produce Figure 1.b). The image is similar to that seen when the plate is placed over a UV transilluminator. b) a plot of the fluorescence volumes against protein concentration and the corresponding correlation coefficient. FluoroProfile is equally suited to use in Xenon-arc based imagers such the BMG Fluostar.

ORDERING

FluoroProfile has been exclusively licensed to SIGMA ALDRICH and can be obtained from your local SIGMA ALDRICH representative or the SIGMA ALDRICH web site.

PROTOCOL

FluoroProfile Protein Quantification Kit Protocol (28K)

INTERFERING COMPOUNDS

Click here for a list of compounds that interfer with the operation of FluoroProfile. (58K)

PRESENTATIONS

New Technology for Protein and Peptide Quantification (122K)

Spectral Characteristics of Epicocconone (122K)

PUBLICATIONS

Click here to view all Fluorotechnics publications.

PRODUCT SUPPORT & SALES

If you would like more information, help or other support from the Fluorotechnics Proteomics Team, please send us a message using the form below.