Fluorotechnics Scientific Publications

FLUOROTECHNICS SELECTED SCIENTIFIC PUBLICATIONS

Journal Articles

Cleemann, F. and Karuso, P. Fluorescence Anisotropy Assay for the Traceless Kinetic Analysis of Protein Digestion. Analytical Chemistry (in press).
This paper describes a new approach to using one of our products LavaDigest to monitor proteolysis.

Nock, C. M., Ball, M. S., White, I. R., Skehel, J. M., Bill, L. and Karuso, P. (2008) Mass Spectrometric Compatibility of Deep Purple and SYPRO Ruby total protein stains for high throughput proteomics using large format two dimensional gel electrophoresis. Rapid Commun. Mass Spect., 22:1-6. available (here 138K )

Karuso, P., Crawford, A. S., Veal, D. A., Scott, G. B. I. and Choi, Y-H. (2008) Real-Time Fluorescence Monitoring of Tryptic Digestion in Proteomics. J. Proteome Res., 7 (1), 361-6. available (here 670K )

Ball, M. S., Karuso, P. (2007) Compatibility of four common fluorescent stains with peptide mass fingerprinting by MALDI-ToF. J. Proteome Res., 6 (11), 4313-20. available (here 241K )

Karuso P., Crawford A. S., Veal, D.A., Scott G. B. I., Choi, H.-Y. (2007) Real-time Fluorescence Monitoring of Tryptic Digestion in Proteomics. Journal of Proteome Research (In-Press)

Choi, H.-Y., Veal, D.A. & Karuso, P. (2006) Epicocconone, A new cell-permeable long stokes’ shift fluorescent stain for live cell imaging and multiplexing. Journal of Fluorescence, 16 (4): 475-482 (available here)

Mackintosh, J.A., Veal, D.A. and Karuso, P. (2005) FluoroProfile, a fluorescence based assay for rapid and sensitive quantification of proteins in solution. Proteomics, 5, 4673-4677.

Coghlan, D. R., Mackintosh, J. & Karuso, P. (2005). Mechanism of reversible fluorescent staining of protein with Epicocconone. Organic Letters, 7, 2401-2404.

Malmport, E., Mackintosh, J., Ji, H., Veal, D. & Karuso, P. (2005). Visualization of proteins electro-transferred on Hybond ECL and Hybond-P using Deep Purple Total Protein Stain. GE Healthcare Life Science News, 19, 12-13.

Mackintosh, J.A., Choi, H.-Y., Bae, S.-H., Veal, D.A., Bell, P.J., Ferrari, B.C., van Dyk, D., Verrills, N.M., Paik, Y.-K. & Karuso, P. (2003). A fluorescent natural product for ultra sensitive detection of proteins in 1-D and 2-D gel electrophoresis. Proteomics, 3, 2273-2288.

Bell, P.J.L. and Karuso, P. (2003) Epicocconone, a novel fluorescent compound from the fungus Epicoccum nigrum. Journal of American Chemical Society, 125, 9304.

Veal, D.A., Bell. P., Brown, H., Choi, H.-Y., Karuso, P. (2003). Fluorophores from fungi. Microbiology Australia, 24 (3): 12-14.

Ferrari, B.C, Attfield P.V. Veal, D.A. and Bell P.J. (2003). Application of the novel fluorescent dye Beljian Red to the differentiation of Giardia cysts. Journal of Microbiological Methods, 52: 133-155.

Bell, P.J.L., Deere, D., Shen, J., Chapman, B., Bissinger, P. H., Attfield, P. V. and Veal, D. A. 1998. Flow cytometric method for rapid selection of novel industrial yeast hybrids. Applied and Environmental Microbiology, 64, 1669-1672.

Conference Abstracts

Cleemann, F., Karuso, P. (2007) Novel fluorescence polarization assay for the traceless enzyme kinetic analysis of protein digestion. RACI NSW Organic Chemistry Group 28th Annual Symposium. December 5, Sydney. p17.

Ball, M. S., Choi, H-Y., Cooney, B., Haynes, P. A., Veal, D. and Karuso, P. (2007) A reversible fluorescent tag for mass spec. compatible detection and quantification of proteins and peptides. KHUPO, 7th Annual International Proteomics Conference, Seoul, Korea, March 29-30, P-93, p136.

Choi, H-Y., Veal, D.A., Crawford, A., Scott, G. and Karuso, P. H. (2007) Traceless fluorescent monitoring of proteolytic digestion. ABRF, Tampa, Florida, USA, March 31-April 3, P136-S, p108-9.

Karuso, P. and Veal D. (2007) Real-time monitoring of proteolytic digestion for shotgun proteomics. HUPO, 6th Annual World Congress, Seoul, Korea, October 6-10, Abstract in Molecular and Cellular Proteomics 6 (8) (suppl.1) HA-0126, p115. ISSN 1535-9476.

Ball, M., Steller, C., Saldanha, R., Hong, J., Simpson, R. and Karuso, P. (2007) A sensitive and simple procedure for Staining proteins on either nitrocellulose of PVDF membranes based on the fluorophore epicocconone. Abstract in: The 12th Lorne Proteomics Symposium, 2-4 February, Lorne Victoria. Abs105, p37. (available here, 327K )

Choi, H-Y., Veal, D.A., Cooney, B., David, S., Karuso, P. (2007). Real-Time Monitoring of Proteolytic Digestion for Shotgun Proteomics. HUPO 6th Annual World Congress, Seoul 2007 October 6 - 10 2007, COEX, Seoul Korea. (available here)

Veal, D.A., Choi, H-Y., Sjöström , S., Cooney, B., David, S., Karuso, P. (2007). Monitoring Proteolytic Digestion using the Fluorophore Epicocconone. ASMS (AMERICAN SOCIETY FOR MASS SPECTROMETRY) 55th Conference. June 3rd - 7th, 2007 Indianapolis, Indiana. (available here, 101K )

Ball, M., Veal, D.A., Karuso, P. (2006). More effective visualization, detection and quantification of glycoproteins using technologies based on the low molecular weight fluorophore epicocconone. HUPO 5th Annual World Congress. October 28 - November 1 2006 Long Beach, California.

Ball, M., Haynes, P.A., Veal, D.A., Karuso, P. (2006). A reversible fluorescent tag for mass spectrometry compatible detection and quantification of proteins and peptides. HUPO 5th Annual World Congress. October 28 - November 1 2006 Long Beach, California.

Ball, M. Veal, D.A., Lyristis, M., Karuso, P. (2006) Application of the fluorophore epicocconone for the improved visualization and quantification of glycoproteins. Peptalk, Coronado California Jan 9 – 13.

Karuso, P. H. Crawford, A. S. Chen, D. E. Mehigh R. J., Geoppo B. S., Scott G. B. , J. Mackintosh, D. A. Veal, H. Choi (2006) Rapid and sensitive fluorescent peptide quantification using epicocconone. Journal of Biomolecular Techniques, 17, (1): P157-S.

Choi H-Y, Crawford A. S., Chen D. E., Mehigh R. J. Wildsmith J. Ray K. M.,. Scott G. B., Mackintosh J., Veal D. A., Karuso P. H. (2006) Real-time monitoring of tryptic digests using the fluorophore epicocconone. Journal of Biomolecular Techniques, 17, (1): P202-S.

Veal, D.A., Mackintosh, J.A., Coghlan, D., Choi. Y. and Karuso, P. (2005) Reversible covalent binding of the fluorophore epicocconone to proteins provides a novel approach to the sensitive quantification of proteins across a wide variety of different platforms. Enabling Technologies for Proteomics Meeting Calgary 22 – 23rd September 2005.

Mackintosh, J., Veal, D., Coghlan, D., Choi, Y., Karuso P. (2005) Application of epicocconone in fluorescence-based protein detection and quantification, East Coast Protein Meeting, June 24-26, 2005, Coffs Harbour. Abstract O19.

Veal, D.A., Mackintosh, J.A., Coghlan, D.R., Choi, H-Y., Karuso, P. (2005) Epicocconone the safe ethidium bromide of proteomics. HUPO 4th Annual World Congress. August 29 – September 1 2005 Munich, Germany. Abstract in Molecular and Cellular Proteomics, 4 (8) (suppl.1) Abstract number 39.56 p S380.

Mackintosh, J., Coghlan, D., Choi, Y., Karuso P., and Veal, D. (2005) Reversible covalent binding of the fluorophore epicocconone to proteins provides a novel approach to the sensitive quantification of proteins across a wide variety of different platforms. 9th Annual Proteomics: Applications in Therapeutics and Diagnostics, Beyond Genome Meeting, June 15-16, 2005 , San Francisco , USA , Abstract No. 308.

Mackintosh, J., Coghlan, D., Choi, Y., Karuso P., and Veal, D. (2005) Reversible Covalent Binding of the Fluorophore Epicocconone to Proteins Provides a Novel Approach to the Sensitive Quantification of Proteins Across a Wide Variety of Different Platforms. Pittcon, Orlando Florida. February 26 to March 4, 2005. Abstract no. 10-39.

Veal, D.A., Mackintosh, J.A., Coghlan, D., Choi. Y. and Karuso, P (2005). Reversible covalent binding of the fluorophore Epicocconone to proteins provides a novel approach to the sensitive quantification of proteins across a wide variety of different platforms. US HUPO-2005 Mapping the Proteome: From Tools to Functionality . March 12-16, 2005 . Arlington , USA . Abstract L-501.

Veal, D.A., Choi, H.Y., Karuso, P.H. (2005). Epicocconone, a new cell-permeable fluorophore for staining cytoplasmic constituents of live cells. US HUPO-2005 Mapping the Proteome: From Tools to Functionality. March 12-16, 2005. Arlington, USA . Abstract P-625.

Veal, D.A., Mackintosh, J., Coghlan, D, Choi, Y. and Karuso, P. (2005). Applications of a new fluorophore, epicocconone, to proteomic discovery. Abstract in: PEPTalk. January 10-13, 2005, San Diego California. Pp 108.

Mackintosh, J.A., Veal, D.A., Coghlan, D.R., Choi, H-Y., Karuso, P. (2004). Application of Epicocconone in fluorescent-based proteomic discovery. Molecular and Cellular Proteomics 3 (10, Supplement), Session 26.2, S293. Abstracts from the HUPO 3rd Annual World Congress, October 25-27 2004 , Beijing .

Xu, N., Mackintosh, J., Karuso, P., Veal, D., Wang, Y., Cordwell, S. and Baker, M. (2004).Visualizing more of the cellular proteome: combining subcellular fractionation with a new fluorescent gel stain. In: Molecular and Cellular Proteomics 3 (10, Supplement), Session 28.19, S330. Abstracts from the HUPO 3rd Annual World Congress, October 25-27 2004, Beijing .

Xu, N., Veal, D., Karuso, P., Mackintosh, J., Wang, Y., Cordwell, S. and Baker, M. (2004). Subcellular proteomic identification of cancer cell proteins modulated by urokinase plasminogen activator receptor using a combination of novel proteomic approaches. In: Abstracts of the XVIIth International Congress on Fibrinolysis and Proteolysis (ISFP). Melbourne , Australia , March 21-25 2004.

Veal, D.A., Mackintosh, J.A., Choi, H-Y., Bae, S-H. and Karuso, P. (2003). Picogram Detection of Proteins in SDS-PAGE using lightning fast protein gel stain. Extract published in: International Congress on Protein Expression and Protein Function Abstracts, Berlin, Germany, May 25-28, 2003. p65

Veal, D.A., Mackintosh, J.A., Choi, H-Y., Bae, S-H. and Karuso, P. (2003). Picogram Detection of Proteins in SDS-Page using Lightning Fast Protein Gel Stain. Extract published in: KHUPO 3rd Annual International Proteomics Conference. Proteomics: Recent Advances in New Technology and It's Applications . Seoul, Korea , March 27-28, 2003 . p17.

Mackintosh, J.A. Choi, H-Y, Bae, S-H., Ferrari, B.C., Karuso, P. H. and Veal D.A. (2002). A new, fast, sensitive fluorescent protein gel stain for proteomic application. Poster and abstract presented at ComBio, 29th September - 3rd October 2002 , Sydney Convention Centre, Sydney , Australia . Australian Society for Biochemistry and Molecular Biology, Australian and New Zealand Society for Cell and Developmental Biology and Australian Society for Plant Scientists.